1 With Slonomics, a defined number of standardized building blocks is chemically synthesized as single-stranded DNA strands containing self-complementary regions. Intra-strand base pairing of these regions leads to the formation of a stable hairpin-like secondary structure comprising a short loop, a double-stranded stem region assuring the stability of the molecule (constant region), and a three-nucleotide single-stranded overhang (variable region). Two different classes of building blocks are defined as “splinkers” and “anchors”.
All splinker molecules share the same structure and differ only in their variable three-base single-stranded overhangs. In contrast, the anchor oligonucleotides differ in the overhang and also in the directly adjacent trinucleotide.
In order to create a library representing all possible permutations, 64 (43) different splinkers and 4096 (46) different anchor oligonucleotides are required.
Each anchor molecule harbors an additional biotin group in the loop region, allowing the oligonucleotide to be coupled to a surface.